Skip to main content

Biochemistry II

Study Course Description

Course Description Statuss:Approved
Course Description Version:7.00
Study Course Accepted:07.08.2024 10:14:32
Study Course Information
Course Code:CFUBK_013LQF level:Level 7
Credit Points:4.00ECTS:6.00
Branch of Science:Clinical MedicineTarget Audience:Life Science
Study Course Supervisor
Course Supervisor:Andrejs Šķesters
Study Course Implementer
Structural Unit:Department of Human Physiology and Biochemistry
The Head of Structural Unit:
Contacts:Riga, 16 Dzirciema Street, cfbkatrsu[pnkts]lv, +371 67061550
Study Course Planning
Full-Time - Semester No.1
Lectures (count)10Lecture Length (academic hours)2Total Contact Hours of Lectures20
Classes (count)7Class Length (academic hours)4Total Contact Hours of Classes28
Total Contact Hours48
Study course description
Preliminary Knowledge:
Medical Biochemistry and Normal Physiology RSU MF II course level.
Objective:
To provide knowledge of the biochemistry of oxygen and nitrogen derivatives (active oxygen and nitrogen of non-radical nature and radicals).
Topic Layout (Full-Time)
No.TopicType of ImplementationNumberVenue
1The concept of O2 as a toxic gas. Introduction to O2 toxicity and active oxygen species (AOS).Lectures1.00auditorium
2Free radical chemistry. Free radical chemistry in relation to AOS. Free radical chemistry, ASF and their relationship with the metabolic processes in the body.Lectures1.00auditorium
3Free radicals, other active forms (nitrogen radicals), as derivatives of biochemical processes in the body. AOS as biomolecules.Lectures1.00auditorium
4Free radicals, active forms of oxygen and nitrogen, their antioxidative regulation and protection.Lectures1.00auditorium
5Oxidative stress, its types: adaptation, damage, repair and cell death.Lectures1.00auditorium
6Local stress in organs and organ systems. Correction options.Lectures1.00auditorium
7Local stress in organs and organ systems. Correction options.Lectures1.00auditorium
8Oxidative stress and antioxidant defense: how does it work in practice?Lectures1.00auditorium
9Nutrition (dietary supplements) as a chronic stress regulator.Lectures1.00auditorium
10Quantitative and qualitative determination of active forms of oxygen and nitrogen and free radicals in the laboratory. Apparatus that can be used to measure markers of stress and indicators of antioxidant defense.Lectures1.00auditorium
11Total polyphenol; Quantification of DPPH and FRAP in biological materials.Classes1.00laboratory
12Determination of Total Antioxidative Status in biological materials.Classes1.00laboratory
13Determination of ABTS in biological materials.Classes1.00laboratory
14Determination of selenium in blood plasma.Classes1.00laboratory
15Determination of GSH or GSH/GSSG in biological materials.Classes1.00laboratory
16Determination of Hb in whole blood and erythrocyte lysate.Classes1.00laboratory
17Determination of MDA in biological materials.Classes1.00laboratory
Assessment
Unaided Work:
Independent study of theoretical topics using other sources, such as scientific books, publications. In order to evaluate the quality of the study course as a whole, the student must fill out the study course evaluation questionnaire on the Student Portal.
Assessment Criteria:
Participation in practical lessons, solving tasks. In the final exam, a scientific work, using the acquired knowledge, on problem studies.
Final Examination (Full-Time):Exam (Written)
Final Examination (Part-Time):
Learning Outcomes
Knowledge:Upon successful completion of the study course, the student will be able to: 1) Tell about the formation and activity of active oxygen and nitrogen species and radicals. 2) Define knowledge of antioxidants, essential trace elements, their role in the norm, changes in risk groups, and in more common diseases and pathological conditions. 3) Understand the analytical equipment used in laboratory practice and interpret modern, standardised analytical methods, inform about the quality control of analyses regulated by existing legislation.
Skills:Having mastered the study course, the student will apply the acquired theoretical knowledge in practice: They will be able to explain: • The formation of oxidants in the body; • Changes in antioxidants and trace elements in risk groups, in cases of common diseases and pathologies. • The student will be able to evaluate the equipment used in laboratory practice and explain the obtained results. • The student will be able to evaluate the quality control of analyses regulated by legislation.
Competencies:On successful completion of this course, students will understand free radical biochemistry, pro-oxidative and antioxidative processes in the body, and the links between these processes and various pathological conditions and diseases, and will be prepared for independent research work in the radical biochemistry.
Bibliography
No.Reference
Required Reading
1Barry Halliwel and John M. C. Gutteridge. Free radicals in Biology and medicine. 5th ed. 2015., pp.936.
2Donald Armstrong, eds. Free Radical and Antioxidant Protocols., 1998, pp.455. (akceptējams izdevums)
3Donald Armstrong, eds. Free radicals in Diagnostic Medicine., 1994, pp.454. (akceptējams izdevums)
4G. Tirzītis, A. Šķesters. Skābekļa atvasinājumu un brīvo radikāļu bioķīmiskie aspekti bioloģijā. 2007, lpp.105. (akceptējams izdevums)
5Olle Selenius, Brian Alloway, Jose A. Centeno et al. Essentials of Medical Geology. Impacts of the Natural Environment on Public Health. ELSEVIER Acad. Press. 2005., pp. 812. (akceptējams izdevums)
6Redox Biochemistry. Eds. Ruma Banerjee; Donald Becker; Martin Dickman; Vadim Gladishev and Stephen Ragsdale, A. John Willey&sons, Inc., Publication
7Clinical Biochemistry. Metabolic and clinical aspects. 2nd edition, Eds by William J. Marshall; Stephen K. Bangert
Additional Reading
1Pub Med un citas datu bāzes.