Biochemistry II

Study Course Description

Course Description Statuss:Approved

Course Description Version:6.00

Study Course Accepted:01.08.2022 15:54:34

Study Course Information
Course Code:		CFUBK_013		LQF level:		Level 7
Credit Points:		4.00		ECTS:		6.00
Branch of Science:		Clinical Medicine		Target Audience:		Life Science
Study Course Supervisor
Course Supervisor:		Andrejs Šķesters
Study Course Implementer
Structural Unit:		Department of Human Physiology and Biochemistry
The Head of Structural Unit:
Contacts:		Riga, 16 Dzirciema Street, cfbkrsu[pnkts]lv, +371 67061550
Study Course Planning
Full-Time - Semester No.1
Lectures (count)		10	Lecture Length (academic hours)		2	Total Contact Hours of Lectures		20
Classes (count)		7	Class Length (academic hours)		4	Total Contact Hours of Classes		28
Total Contact Hours								48
Study course description
Preliminary Knowledge:
Medical Biochemistry and Normal Physiology RSU MF II course level.
Objective:
To provide knowledge of the biochemistry of oxygen and nitrogen derivatives (active oxygen and nitrogen of non-radical nature and radicals).
Topic Layout (Full-Time)
No.	Topic			Type of Implementation		Number	Venue
1	The concept of O2 as a toxic gas. Introduction to O2 toxicity and active oxygen species (AOS).			Lectures		1.00	auditorium
2	Free radical chemistry. Free radical chemistry in relation to AOS. Free radical chemistry, ASF and their relationship with the metabolic processes in the body.			Lectures		1.00	auditorium
3	Free radicals, other active forms (nitrogen radicals), as derivatives of biochemical processes in the body. AOS as biomolecules.			Lectures		1.00	auditorium
4	Free radicals, active forms of oxygen and nitrogen, their antioxidative regulation and protection.			Lectures		1.00	auditorium
5	Oxidative stress, its types: adaptation, damage, repair and cell death.			Lectures		1.00	auditorium
6	Local stress in organs and organ systems. Correction options.			Lectures		1.00	auditorium
7	Local stress in organs and organ systems. Correction options.			Lectures		1.00	auditorium
8	Oxidative stress and antioxidant defense: how does it work in practice?			Lectures		1.00	auditorium
9	Nutrition (dietary supplements) as a chronic stress regulator.			Lectures		1.00	auditorium
10	Quantitative and qualitative determination of active forms of oxygen and nitrogen and free radicals in the laboratory. Apparatus that can be used to measure markers of stress and indicators of antioxidant defense.			Lectures		1.00	auditorium
11	Total polyphenol; Quantification of DPPH and FRAP in biological materials.			Classes		1.00	laboratory
12	Determination of Total Antioxidative Status in biological materials.			Classes		1.00	laboratory
13	Determination of ABTS in biological materials.			Classes		1.00	laboratory
14	Determination of selenium in blood plasma.			Classes		1.00	laboratory
15	Determination of GSH or GSH/GSSG in biological materials.			Classes		1.00	laboratory
16	Determination of Hb in whole blood and erythrocyte lysate.			Classes		1.00	laboratory
17	Determination of MDA in biological materials.			Classes		1.00	laboratory
Assessment
Unaided Work:
Independent study of theoretical topics using other sources, such as scientific books, publications.
Assessment Criteria:
Participation in practical lessons, solving tasks. A written multiple-choice question test at the end-of-course examination.
Final Examination (Full-Time):		Exam (Written)
Final Examination (Part-Time):
Learning Outcomes
Knowledge:		Upon successful completion of the study course, the student will be able to: 1) Tell about the formation and activity of active oxygen and nitrogen species and radicals. 2) Define knowledge of antioxidants, essential trace elements, their role in the norm, changes in risk groups, and in more common diseases and pathological conditions. 3) Understand the analytical equipment used in laboratory practice and interpret modern, standardised analytical methods, inform about the quality control of analyses regulated by existing legislation.
Skills:		Having mastered the study course, the student will apply the acquired theoretical knowledge in practice: They will be able to explain: • The formation of oxidants in the body; • Changes in antioxidants and trace elements in risk groups, in cases of common diseases and pathologies. • The student will be able to evaluate the equipment used in laboratory practice and explain the obtained results. • The student will be able to evaluate the quality control of analyses regulated by legislation.
Competencies:		On successful completion of this course, students will understand free radical biochemistry, pro-oxidative and antioxidative processes in the body, and the links between these processes and various pathological conditions and diseases, and will be prepared for independent research work in the radical biochemistry.
Bibliography
No.	Reference
Required Reading
1	Barry Halliwel and John M. C. Gutteridge. Free radicals in Biology and medicine. 5th ed. 2015., pp.936.
2	Donald Armstrong, eds. Free Radical and Antioxidant Protocols., 1998, pp.455. (akceptējams izdevums)
3	Donald Armstrong, eds. Free radicals in Diagnostic Medicine., 1994, pp.454. (akceptējams izdevums)
4	G. Tirzītis, A. Šķesters. Skābekļa atvasinājumu un brīvo radikāļu bioķīmiskie aspekti bioloģijā. 2007, lpp.105. (akceptējams izdevums)
5	Olle Selenius, Brian Alloway, Jose A. Centeno et al. Essentials of Medical Geology. Impacts of the Natural Environment on Public Health. ELSEVIER Acad. Press. 2005., pp. 812. (akceptējams izdevums)
6	Redox Biochemistry. Eds. Ruma Banerjee; Donald Becker; Martin Dickman; Vadim Gladishev and Stephen Ragsdale, A. John Willey&sons, Inc., Publication
7	Clinical Biochemistry. Metabolic and clinical aspects. 2nd edition, Eds by William J. Marshall; Stephen K. Bangert
Additional Reading
1	Pub Med un citas datu bāzes.