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Toxicological Chemistry

Study Course Description

Course Description Statuss:Approved
Course Description Version:1.00
Study Course Accepted:21.03.2024 13:20:47
Study Course Information
Course Code:FKK_051LQF level:Level 7
Credit Points:4.67ECTS:7.00
Branch of Science:Basic Sciences of Medicine, including PharmacyTarget Audience:Pharmacy
Study Course Supervisor
Course Supervisor:Katrīna Sudmale
Study Course Implementer
Structural Unit:Department of Pharmaceutical Chemistry
The Head of Structural Unit:
Contacts:Riga, 16 Dzirciema Street, Block A, 5th floor, Room no 502, farmkkatrsu[pnkts]lv, +371 67061544
Study Course Planning
Full-Time - Semester No.1
Lectures (count)6Lecture Length (academic hours)2Total Contact Hours of Lectures12
Classes (count)5Class Length (academic hours)4Total Contact Hours of Classes20
Total Contact Hours32
Full-Time - Semester No.2
Lectures (count)0Lecture Length (academic hours)0Total Contact Hours of Lectures0
Classes (count)11Class Length (academic hours)4Total Contact Hours of Classes44
Total Contact Hours44
Part-Time - Semester No.1
Lectures (count)8Lecture Length (academic hours)4Total Contact Hours of Lectures32
Classes (count)8Class Length (academic hours)4Total Contact Hours of Classes32
Total Contact Hours64
Study course description
Preliminary Knowledge:
Inorganic, organic and analytical chemistry, human anatomy, physiology and pathology.
Objective:
• to promote the acquisition of knowledge on the main methods of analysis for the determination of toxic substances in biological objects • to promote awareness of the possibilities of chemical and physico-chemical analysis methods in toxicological analysis • to master the types of tasks of the issues included in the course and the methodology of their solution
Topic Layout (Full-Time)
No.TopicType of ImplementationNumberVenue
1Toxicology and analytical toxicology. Dose-response relationship.Lectures1.00auditorium
2Analytical toxicology of volatile toxicants.Lectures1.00auditorium
3Physio-chemical properties and methods of isolation of non-volatile organic toxicants.Lectures1.00auditorium
4Chromatographic and spectral methods in analysis of drugs and other organic toxicants.Lectures1.00auditorium
5Analytical toxicology of toxic cations, anions and pesticides.Lectures1.00auditorium
6Essentials of toxicokinetics and toxicodynamics for interpretation of analytical results.Lectures1.00auditorium
7Isolation and identification of volatile toxicants. Analysis of volatile toxicants.Classes0.50laboratory
8Colloquium. General principles of analytical toxicology. Volatile toxicants.Classes1.00laboratory
9Isolation of non volatile organic toxicants. Analysis of drugs in biological samples.Classes0.50laboratory
10Colloquium. Isolation and analysis of non-volatile organic toxicants.Classes1.00laboratory
11Identification of non-volatile organic toxic substances.Classes0.50laboratory
12Colloquium. Chemical-toxicological analysis of poisonous cations, anions and pesticides.Classes1.00laboratory
13Mineralization of biological material.Classes0.50laboratory
14Isolation and analysis of volatile toxic compounds. Control analysis (volatile poisons).Classes0.50laboratory
15Isolation of non-volatile organic toxicants.Classes0.50laboratory
16Identification of non-volatile organic toxicants. Control analysis (non-volatile organic poisons).Classes0.50laboratory
17Isolation and demonstration of non-volatile organic toxicants.Classes0.50laboratory
18Detection of toxic cations in minerals. Control analysis (metallic poisons).Classes0.50laboratory
19Detection of toxic anions. Control analysis.Classes0.50laboratory
20Control analysis: Isolation and demonstration of volatile compounds. Full forensic chemical analysis.Classes1.00laboratory
21Control analysis: Isolation and demonstration of drug substances.Classes0.50laboratory
22Control analysis: isolation and detection of toxic cations.Classes0.50laboratory
23Colloquium: Isolation and identification of toxicants.Classes0.50laboratory
24Detection of toxic compounds in urine by colour reactions. Control analysis: Express methods in the diagnosis of poisoning.Classes1.00laboratory
25Detection of drug substances in urine by solid phase extraction and thin layer chromatography screening. Control test: Identification of the toxic compound.Classes1.00laboratory
26Detection of therapeutic substances in urine by extraction and electron spectroscopy. Control analysis: Identification of toxicants in urine by their UV spectra.Classes1.00laboratory
27Seminar on toxicokinetics. Test on toxicokinetics.Classes0.50laboratory
28Seminar on toxicant metabolism. Test on metabolism.Classes0.50laboratory
29Colloquium: Toxicokinetics and toxicodynamics.Classes0.50laboratory
30Use of infrared spectroscopy for identification of toxicants. Control analysis: Identification of drug substances by IS spectra.Classes1.00laboratory
Topic Layout (Part-Time)
No.TopicType of ImplementationNumberVenue
1Toxicology and analytical toxicology. Dose-response relationship.Lectures1.00auditorium
2Analytical toxicology of volatile toxicants.Lectures1.50auditorium
3Physio-chemical properties and methods of isolation of non-volatile organic toxicants.Lectures1.50auditorium
4Chromatographic and spectral methods in analysis of drugs and other organic toxicants.Lectures1.50auditorium
5Analytical toxicology of toxic cations, anions and pesticides.Lectures1.00auditorium
6Essentials of toxicokinetics and toxicodynamics for interpretation of analytical results.Lectures1.50auditorium
7Isolation and identification of volatile toxicants. Analysis of volatile toxicants.Classes0.50laboratory
8Colloquium. General principles of analytical toxicology. Volatile toxicants.Classes0.50laboratory
9Isolation of non volatile organic toxicants. Analysis of drugs in biological samples.Classes0.50laboratory
10Colloquium. Isolation and analysis of non-volatile organic toxicants.Classes0.50laboratory
11Identification of non-volatile organic toxic substances.Classes0.50laboratory
12Colloquium. Chemical-toxicological analysis of poisonous cations, anions and pesticides.Classes0.50laboratory
13Mineralization of biological material.Classes1.00laboratory
14Isolation and analysis of volatile toxic compounds. Control analysis (volatile poisons).Classes0.50laboratory
15Isolation of non-volatile organic toxicants.Classes0.50laboratory
19Detection of toxic anions. Control analysis.Classes0.50laboratory
20Control analysis: Isolation and demonstration of volatile compounds. Full forensic chemical analysis.Classes0.50laboratory
24Detection of toxic compounds in urine by colour reactions. Control analysis: Express methods in the diagnosis of poisoning.Classes0.50laboratory
26Detection of therapeutic substances in urine by extraction and electron spectroscopy. Control analysis: Identification of toxicants in urine by their UV spectra.Classes0.50laboratory
28Seminar on toxicant metabolism. Test on metabolism.Classes0.50laboratory
29Colloquium: Toxicokinetics and toxicodynamics.Classes0.50laboratory
Assessment
Unaided Work:
Individual work in the laboratory, performing experiments, solving situation tasks. It is recommended to fill in the study course survey, evaluating the existing course content and creating proposals for updating the course.
Assessment Criteria:
1. Colloquia and seminars on toxic effects, their isolation, analysis, data interpretation. (30%) 2. Solving situation tasks. (10%) 3. Practical isolation of toxicants from biological objects, their analysis and interpretation of results. (10%) 4. Exam: knowledge of the mechanisms of action of toxicants, their isolation from the section material, qualitative and quantitative analysis, the use of regularities of toxicokinetics and toxicodynamics in interpreting the results of analyses are tested. (50%)
Final Examination (Full-Time):Exam (Written)
Final Examination (Part-Time):Exam (Written)
Learning Outcomes
Knowledge:After completing the study course, students will be able to: • evaluate the objects to be used in the toxicological analysis; • present the theoretical basis of the methods used in the analysis; • understand and interpret analytical methodologies available in the literature.
Skills:As a result of mastering the study course students: • will be able to isolate different types (volatile, organic, non-volatile, metal-containing) toxicants from biological objects; • will be able to use chromatographic, spectroscopic methods and chromogenic reactions in an experiment to detect the most well-known toxicants; • will be able to apply toxicokinetics and toxicodynamics to the interpretation of the results of legality analyses.
Competencies:As a result of the study course, students will be able to isolate the most well-known toxicants from biological objects, analyse and interpret the results.
Bibliography
No.Reference
Required Reading
1Casarett and Doull's toxicology: the basic science of poisons / editor, Curtis D. Klaassen. 9th ed. New York: McGraw-Hill Education, 2019. xiii, 1454 lpp.
2V.Liguts. Toksikoloģijas rokasgrāmata. Rīga, 2001. (akceptējams izdevums)
3S.Jickels, A.Negrusz (eds). Clarke’s Analytical Forensic Toxicology. 2nd ed. Pharmaceutical Press, London, Chicago, 2013.
4R.J.Flanagan, et al. Fundamentals of Analytical Toxicology. Willey, 2020
5Ārvalstu studentiem/For international students
6Casarett and Doull's toxicology: the basic science of poisons / editor, Curtis D. Klaassen. 9th ed. New York: McGraw-Hill Education, 2019. xiii, 1454 lpp.
7S.Jickels, A.Negrusz (eds). Clarke’s Analytical Forensic Toxicology. 2nd ed. Pharmaceutical Press, London, Chicago, 2013.
Additional Reading
1J.A.Timbrell. Introduction to Toxicology. London, 2009
2A. C. Moffat, et al. (eds). Clarke’s Analysis of Drugs and Poisons. London, Chicago, 2011.