Scientific Laboratory of Molecular Genetics

Accredited medical laboratory according to ISO:15189 standards with an LATAK-633-2020 accreditation certificate, within the framework of which part of its examinations are accredited.

The Scientific Laboratory of Molecular Genetics conducts research on the genetic causes of diseases and their characteristics, especially in the field of rare diseases, the use of biomarkers in diagnostics, and prognosis of the course of diseases.

The laboratory provides students, residents, and doctoral students with the opportunity to develop scientific research.

The laboratory cooperates with various partners both in Latvia (the genome database of the Latvian population, Riga Eastern Clinical University Hospital, the Children's Clinical University Hospital etc.) and abroad (the Academy of Medical Sciences of Ukraine, the Institute of Hereditary Pathology and more).

Research

Research Areas

• Rare monogenic disorders
  • Cystic fibrosis (mucoviscidosis)
  • Wilson disease
  • Apha-1-antitrypsin deficiency
  • Kennedy disease
  • Ornithine transcarbamilase deficit
  • Crigler0Najjar syndrom etc.

• Monogenic pathologies
  • Gilbert syndrome
  • HFE-related hereditary haemochromatosis
• Multifactorial pathologies
  • Infertility
  • Myeloproliferative disorders (JAK2, CALR, MPL gene allelic variants)

Research is conducted with permission from the Central Medical Ethics Committee.

Services

Technologies

• DNA isolation from different biological materials
• Quantitative and qualitative measurements of DNA quality
• Sequencing service:
  • Direct sequencing
  • Fragment analysis / microsatellite analyses
• Genotyping
  • Direct sequencing
  • PCR-RFLP, ARMS-PCR
  • qPCR, HRM, TaqMan

Pathogenic varint detection in following disorders

• Cystic fibrosis (most frequent causative variant and whole CFTR gene sequencing)
• Wilson's disease (most frequent causative variant and whole ATP7B gene sequencing)
• Myeloproliferative neoplasia (JAK2  gene V617F variant, CALR gene  9th exon ind/del analysis)
• Inherited thrombophilia (F5 gene G1691A variant, F2 (protrombine) gene G20210A variant, MTHFR gene C677T and A1298C variant analysis)
• Male infertility (Y chromosome microdeletion analysis)

Projects

ERDF

• ERDF project No. 2014/0048/2DP/2.1.1.1.0/14/APIA/VIAA/074. Development of a new method for the detection of genetic variations significant in reproduction. Project manager: I. Kempa. Duration: 2014-2015
• ERDF project No KC-PI-2017/24 Test for detection of infertility genetic causes. Project manager: L. Gailīte. Duration: 2017-2020

Grants by the Latvian Council of Science & Fundamental and Applied Research Projects

• Project number: lzp-2020/1-0042. Elucidating comprehensive etiology of cervical insufficiency to foster timely diagnosis of preterm delivery and prevent adverse outcomes in obstetrics.
  Project manager: Dace Rezeberga, Duration: 2021–2023
• Project number: lzp-2020/1-0269. Predominantly primary antibody deficiencies among adults: solving etiology and causes of clinical variability.
  Project manager: Assoc.Prof. Nataļja Kurjāne, Duration: 2021-2023
• Project number: lzp-2020/2-0275. Pilot study: newborn screening for spinal muscular atrophy.
  Project manager: Madara Kreile, Duration: 2020-2021
• Project number: lzp-2020/2-0111. Investigating the Role of Genome Instability in Male Infertility.
  Project manager:  Juris Ērenpreiss, Duration: 2020-2021
• Implementation of the sub-project “Polymorphic variants of xenobiotic metabolism genes in the Latvian population and their relation to pathology” of the Latvian Council of Science project No. 10.0010 “Genetic research of diseases etiology, pathogenesis, and human ageing processes of the Latvian population”. Duration: 2010–2013
• Latvian Council of Science Grant No. 09.1384. "Expression of genetically determined modulations of liver metabolism in cases of different hepatic pathologies." Duration: 2009–2012
• Latvian Council of Science Grant No. 09.1116. "Diagnosis of human pathology caused by mitochondrial DNA mutations and analysis of etiopathogenesis." Duration: 2009–2012

• Latvian Council of Science Grant No. 09.1115. “Importance of risk factors in the development of non-syndromic cleft palate, cleft lip with/without cleft palate for the Latvian population”. Duration: 2009–2011

Publications

2021

• Dangouloff T., Vrscaj E., Servais L., Osredkar D., The SMA NBS World study group (including Kreile M.). "Newborn screening programs for spinal muscular atrophy worldwide: Where we stand and where to go." Neuromuscular Disorders. 2021, 31(6):574-582. https://doi.org/10.1016/j.nmd.2021.03.00
• Moisejevs G., Bormane E., Trumpika D., Baufale R., Busmane I., Voicehovska J., Grigane A., Suba O., Silova A., Skesters A., Lejnieks A., Gailite L. "Glutathione reductase is associated with the clinical outcome of septic shock in the patients treated using continuous veno-venous haemofiltration." Medicina (Lithuania). 2021, 57(7):689.  https://doi.org/10.3390/medicina57070689
• Puzuka A., Alksere B., Gailite L., Erenpreiss J. "Idiopathic Infertility as a Feature of Genome Instability." Life. 2021, 11(7):628 https://doi.org/10.3390/life11070628
• Millere E., Rots D., Simren J., Ashton N.J. Kupats E., Micule I., Priedite V., Kurjane N., Blennow K., Gailite L., Zetterberg H., Kenina V. "Plasma neurofilament light chain as a potential biomarker in Charcot-Marie-Tooth disease." European Journal of Neurology. 2021, 28(3):974-981. https://doi.org/10.1111/ene.14689
• Millere E., Rots D., Glazere I., Taurina G., Kurjane N., Priedite V., Gailite L., Blennow K., Zetterberg H., Kenina V. "Clinical Phenotyping and Biomarkers in Spinal and Bulbar Muscular Atrophy." Front. Neurol. 2021, https://doi.org/10.3389/fneur.2020.586610
• Kovale S., Terauda R., Millere E., Taurina G., Murmane D., Isakova J., Kenina V., Gailite L. "GJB1 Gene Analysis in Two Extended Families with X-Linked Charcot-Marie-Tooth Disease." Case series – General Neurology. 2021, 13:422-428. https://doi.org/10.1159/000515170
• Lidaka L., Berkere L., Lazdane G., Dzivite-Krisane I., Kivite-Yrtane A., Gailite L. "Non-Classical Congenital Adrenal Hyperplasia-Causing Alleles in Adolescent Girls with PCOS and in Risk Group for PCOS Development." Diagnostics. 2021, 11(6):980  https://doi.org/10.3390/diagnostics11060980
• Lidaka L., Grasmane A., Lazdane G., Dzivite-Krisane I., Gailite L., Viberga I. "Can a mother’s polycystic ovary syndrome (PCOS)-related symptoms be used to predict the future clinical profile of PCOS in her adolescent daughter? A pilot study." The European Journal of Contraception and Reproductive Health Care. 2021, 26(1):17-22. https://doi.org/10.1080/13625187.2020.1795118

2020

• Kreile M., Lubina O., Ozola-Zalite I., Lugovska R., Pronina N., Steran O., Vevere P., Konika M., Malniece I., Gailite L. "Phenylketonuria in the Latvian population: Molecular basis, phenylanlanyne levels, and patient compliance." Molecular Genetics and Metabolism Reports. 2020, 25:100671. DOI: 10.1016/j.ymgmr.2020.100671
• Millere E., Gribuste L., Kazaine I., Strautmanis J., Gailite L., Kenina V. "Clinical and neurophysuological spectrum of polyneuropathies in children." Neulogia i Neurochirurgia Polska. 2020, 54(5):466-470. 10.5603/PJNNS.a2020.0068
• Volozonoka L., Gailite L., Perminov D., Kornejeva L., Fodina V., Kempa I., Miskova A. "Reducing misdiagnosis caused by maternal cell contamination in genetic testing for early pregnancy loss." Systems Biology in Reproductive Medicine 2020, 66(6):410-420. DOI: 10.1080/19396368.2020.1827081
• Millere E., Rots D., Simren J., Ashton NJ, Kupats E., Micule I., Priedite V., Kurjane N., Blennow K., Gailite L., Zetterberg H., Kenina V. European Journal of Neurology, 2020, doi:10.1111/ene.14689
• Rudaka I., Rots, D., Uzars, A., Kalejs, O., Gailite, L. "Association between 4q25 variants, risk of atrial fibrillation and echocardiographic parameters." Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact and Applied Sciences, 2020, 74(1):1-6. doi: 10.2478/prolas-2020-0001
• Makhuh, H., Hayboniuk, I., Zarina, a., Semeriak, O.M., Gailite, L. "Mutations in the ATP7B gene in Ukranian patients with high risk of Wilson disease." Cyology and Genetics, 2020 54(4):324-332. doi: 10.10.3103/S009545272004009X
• Gailite, L., Valenzuela-Palomo, A., Sanoguera-Miralles, L., Rots, D., Kreile M., Velasco, E.A. (2020) "UGT1A1 Variants c.864+5G>T and c.996+2_996+5del of a Crigler-Najjar Patient Induce Aberrant Splicing in Minigene Assays." Frontiers in Genetics, 11(169) doi: 10.3389/fgene.2020.00169
• Lidaka, L., Grasmane, A., Lazdane, G., Dzivite-Krisane, I., Gailite, L., Viberga, I. "Can a mother’s polycystic ovary syndrome (PCOS)-related symptoms be used to predict the future clinical profile of PCOS in her adolescent daughter? A pilot study." European Journal of Contraception and reproductive health Care, 2020. doi. 10.1080/13625187.2020.1795118
• Malniece, I., Grasmane, A., Inashkina, I., Stavusis, J., Kreile, M., Miklasevics, E., Gailite, L. (2020) "The fetal phenotype of Noonan sydnrome caused by severe, cancer-related PTPN11 variants." American Journal of Case Reports 21”: e922468-1-e922468-6. doi: 10.12659/AJCR.922468
• Volozonoka, L., Rots, D., Kempa, I., Kornete, A., Rezeberga, D., Gailite, L., Miskova A. "Genetic landscape of preterm birth duie to cervical insufficiency: comprehensive analysis and patient next generation sequencing data interpretation." Plos One, 2020, 15(3), e0230771, doi: 10.10.1016/j.bpobgyn.2018.03.007
• Nokalna, I., Kreile, M., Butane, D., Kovalova, Z., Daneberga, Z., Miklasevics, E., Gardovska, D., Gailite, L. (2020) "X-linked lymphoproliferative disease in Latvia: a report of two clinically distinct cases." Case Reports in Medicine 2020, article number: 7108657. doi: 10.1155/2020/7108657

2019

• Zarina A., Tolmane I., Krumina Z., Tutane A.I., Gailite L. Association of variants in the CP, ATOX1 and COMMD1 genes with Wilson disease symptoms in Latvia. Balkan J Med Genet 2019; 22(2):37-42. doi: 10.2478/bjmg-2019-0023
• Alksere B., Berzina D., Dudorova A., Conka U., Andersone S., Pimane E., Krasucka S., Blumberga A., Dzalbs A., Grinfelde I., Vedmedovska N., Fodina V., Erenpreiss J. Case of Inherited Partial AZFa Deletion without Impact on Male Fertility. Case Rep Genet 2019; 2019:3802613. doi: 10.1155/2019/3802613
• Salmina K., Gerashchenko BI, Hausmann M., Vainshelbaum NM., Zayakin P., Erenpreiss J., Freivalds T., Cragg MS., Erenpreisa J. When Three Isn't a Crowd: A Digyny Concept for Treatment-Resistant, Near-Triploid Human Cancers. Genes (Basel) 2019; 10(7) 10.3390/genes10070551
• Erenpreiss J., Fodina V., Pozarska R., Zubkova K., Dudorova A., Pozarskis A.  Prevalence of testosterone deficiency among aging men with and without morbidities. Aging Male 2019; 1:1-5. Doi: 10.1080/13685538.2019.1621832
• Hilz, P., Heinrihsone, R., Pätzold, L. A., Qi, Q., Trofimovics, G., Gailite, L., Irmejs, A., Gardovskis, J., Miklasevics, E., Daneberga, Z. Allelic variants of breast cancer susceptibility genes PALB2 and RECQL in the Latvian population. Hered Cancer Clin Pract. 2019 Jul 3;17:17. doi: 10.1186/s13053-019-0116-6.
• Konrade, I., Zavorikina, J., Fridvalde, A., Rots, D., Kalere, I., Strumfa, I., Dambrova, M., Gailite, L. Novel Variant of the Androgen Receptor Gene in a Patient With Complete Androgen Insensitivity Syndrome and Polyorchidism. Front Endocrinol (Lausanne). 2019 Jan 17;9:795. doi: 10.3389/fendo.2018.00795.
• Oud, M. S., Volozonoka, L., Smits, R. M., Vissers, L. E. L. M., Ramos, L., Veltman, J. A. A systematic review and standardized clinical validity assessment of male infertility genes. Hum Reprod. 2019 May 1;34(5):932-941. doi: 10.1093/humrep/dez022
• Grasmane, A., Rots, D., Vitina, Z., Magomedova V., Gailite, L. The association of FMR1 gene (CGG)n variation with idiopathic female infertility. Arch Med Sci 2019; 15. DOI: https://doi.org/10.5114/aoms.2019.85154
• Hilz, P., Heinrihsone, R., Pätzold, L. A., Qi, Q., Trofimovics, G., Gailite, L., Irmejs, A., Gardovskis, J., Miklasevics, E., Daneberga, Z. Allelic variants of breast cancer susceptibility genes PALB2 and RECQL in the Latvian population. Hered Cancer Clin Pract. 2019 Jul 3;17:17. doi: 10.1186/s13053-019-0116-6.
• Konrade, I., Zavorikina, J., Fridvalde, A., Rots, D., Kalere, I., Strumfa, I., Dambrova, M., Gailite, L. Novel Variant of the Androgen Receptor Gene in a Patient With Complete Androgen Insensitivity Syndrome and Polyorchidism. Front Endocrinol (Lausanne). 2019 Jan 17;9:795. doi: 10.3389/fendo.2018.00795.
• Oud, M. S., Volozonoka, L., Smits, R. M., Vissers, L. E. L. M., Ramos, L., Veltman, J. A. A systematic review and standardized clinical validity assessment of male infertility genes. Hum Reprod. 2019 May 1;34(5):932-941. doi: 10.1093/humrep/dez022
• Grasmane, A., Rots, D., Vitina, Z., Magomedova V., Gailite, L. The association of FMR1 gene (CGG)n variation with idiopathic female infertility. Arch Med Sci 2019; 15. DOI: https://doi.org/10.5114/aoms.2019.85154

2018

• Krumina, A., Pliss, L., Zarina, G., Puzuka, A., Zarina, A., Lace, B., Elferts, D., Khrunin, A., Limborska, S., Klovins, J. Gailite, L. Population Genetics of Latvians in the Context of Admixture between North-Eastern European Ethnic Groups. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences Volume 72, Issue 3, 1 June 2018, Pages 131-151. DOI:10.2478/prolas-2018-0025
• Moisejevs, G., Daugule, I., Isajevs, S., Rudzite, D., Janciauskas D., Tolmanis, I., Leja, M. Non-Invasive Diagnosis of Gastroesophageal Reflux Disease Using Gastrin- and Pepsinogen-Based Tests. Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences Volume 72, Issue 3, 1 June 2018, Pages 172-176. DOI: 10.2478/prolas-2018-0028
• Grasmane, A., Purina-Liberte, K., Rots, D., Miltina, I., Rezeberga, D. Association of in-vitro fertilization twin pregnancy with maternal and perinatal complications. Asian Pacific Journal of Reproduction,Volume 7, Issue 4, July 2018, Pages 151-154. DOI: 10.4103/2305-0500.237051
• Kreile, M., Rots, D., Zarina, A., Rautiainen, L., Visnevska-Preciniece, Z., Kovalova, Z. og Gailite, L. (2018). Association of ARID5B Genetic Variants with Risk of Childhood B Cell Precursor Acute Lymphoblastic Leukaemia in Latvia. Asian Pac J Cancer Prev, 19(1), 91-95. doi:10.22034/apjcp.2018.19.1.91
• Rots, D., Kreile, M., Nikulshin, S., Kovalova, Z. og Gailite, L. (2018). Influence of IL15 gene variations on the clinical features, treatment response and risk of developing childhood acute lymphoblastic leukemia in Latvian population. Pediatr Hematol Oncol, 35(1), 37-44. doi:10.1080/08880018.2018.1440334
• Volozonoka, L., Perminov, D., Kornejeva, L., Alksere, B., Novikova, N., Pimane, E. J., Blumberga, A., Kempa, I., Miskova A., Gailite, L., Fodina, V. (2018). Performance comparison of two whole genome amplification techniques in frame of multifactor preimplantation genetic testing. J Assist Reprod Genet. doi:10.1007/s10815-018-1187-4
• Skuja, V., Derovs, A., Pekarska, K., Rudzite, D., Lavrinovica, E., Piekuse, L., Kempa, I., Straume, Z., Eglite, J., Lejnieks, A., Krumina, A., Eliakim, R. (2018). Gut colonization with extended-spectrum beta-lactamase-producing Enterobacteriaceae may increase disease activity in biologic-naive outpatients with ulcerative colitis: an interim analysis. Eur J Gastroenterol Hepatol, 30(1), 92-100. doi:10.1097/meg.0000000000000989

2017

• Zarina, A., Tolmane, I., Kreile, M., Chernushenko, A., Cernevska, G., Pukite, I.,  Micule, I., Krumina, Z., Krumina, A., Rozentale, B., Piekuse, L. (2017). Genetic variation spectrum in ATP7B gene identified in Latvian patients with Wilson disease. Mol Genet Genomic Med, 5(4), 405-409. doi:10.1002/mgg3.297
• Rudaka, I., Rots, D., Uzars, A., Grineviča, Ļ., Kalējs, O., Piekuse, L. (2017) Ģenētisko variantu rs1805127, rs1892593 un rs2200733 asociācija ar ātriju fibrilācijas attīstības risku. RSU Zinātniskie raksti 2016: 239-247
• Bidiņa, L., K. Kupics, E. Sokolova, M. Pavlovičs, Z. Dobele, L. Piekuse, O. Kalējs.  (2017) PKP2 un DSG2 gēnu ģenētisko variāciju patogenitātes izvērtēšana. RSU Zinātniskie raksti 2016: 223-230
• Skuja, V., Krūmiņa, A., Pekarska, K., Straume, Z., Rudzīte, D.,  Lavrinoviča, E., Piekuse, L., Kempa, I., Derovs, A., Lejnieks, A. (2017)  Čūlainā kolīta klīniskās aktivitātes smaguma saistība ar multirezistento paplašināta spektra beta laktamāzi producējošo Enterobacteriaceae dzimtas baktēriju kolonizāciju zarnu traktā: provizoriskie pētījuma dati. RSU Zinātniskie raksti 2016: 82-93

2016

• Kreile, M., Piekuse, L., Rots, D., Dobele, Z., Kovalova, Z. og Lace, B. (2016). Analysis of possible genetic risk factors contributing to development of childhood acute lymphoblastic leukaemia in the Latvian population. Arch Med Sci, 12(3), 479-485. doi:10.5114/aoms.2016.59920
• Skuja, V., Pekarska, K., Caune, U., Piekuse, L., Kempa, I., Rudzīte, D., Kigitoviča, D., Derovs, A., Vīksna, L., Lejnieks, A., & Krūmiča, A. (2016). Risk Factors that Determine Less Favourable Hospitalisation Course and Outcome in Patients with ESBL Producing Enterobacteriaceae Infection: Preliminary Results / Riska Faktori, Kas Nosaka Sliktāku Hospitalizācijas Gaitu Un Iznākumu Pacientiem Ar Esbl Producējošu Enterobacteriaceae Dzimtas Baktēriju Infekciju: Sākotnējie Rezultāti, Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., 70(4), 252-255. doi: https://doi.org/10.1515/prolas-2016-0025
• Martinsone, Ž., Pilmane, M., Moisejevs, G., Sprūdža, D., & Baķe, M. (2016). Evaluation of Histological Findings of Airways of Rats Exposed to Highly Polluted Indoor Air in Offices, Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences., 70(5), 341-344. doi: https://doi.org/10.1515/prolas-2016-0052
• Inashkina I, Jankevics E, Stavusis J, Vasiljeva I, Viksne K, Micule I, Strautmanis J, Naudina MS, Cimbalistiene L, Kucinskas V, Krumina A, Utkus A, Burnyte B, Matuleviciene A, Lace B. Robust genotyping tool for autosomal recessive type of limb-girdle muscular dystrophies BMC Musculoskelet Disord. 2016 May 4;17:200. doi: 10.1186/s12891-016-1058-z.
• Stavusis, J., Inashkina, I., Lace, B., Pelnena, D., Limborska, S., Khrunin, A., Kucinskas, V., Krumina, A., Piekuse, L., Erenpreiss, J. (2016). A New Baltic Population-Specific Human Genetic Marker in the PMCA4 Gene. Hum Hered, 82(3-4), 140-146. doi:10.1159/000481434
• Luize Bidina, Kaspars Kupics, Emma Sokolova, Mihails Pavlovic, Zane Dobele, Linda Piekuse, Oskars Kalejs. A case report: PKP2 gene c.1592t>g variation in homozygous form identified in arrhythmogenic right ventricular dysplasia patient. CBU International Conference Proceedings, 2016, 631-633.
• L. Piekuse, I. Kempa, D. Rudzite, A. Lejnieks, A. Krumina. Digestive System Diseases as the most Common Disease Group among Patients with Extended-spectrum Beta-lactamase Producing Bacterial Infection. RSU Collectio of Scientific Papers: Research articles in medicine & pharmacy, 2015: Internal Medicine. Surgery. Medical Basic Sciences. Stomatology. Pharmacy. – Rīga: Rīga Stradiņš University, 2016. Pp.5-16
• Piekuse L, Šteinberga Z, Liaviņa L, Krūmiņa A, Kempa I. Jaunas metodes izveide un aprobācija ar asinsreces traucējumiem saistītu reprodukcijai nozīmīgu ģenētisko variāciju noteikšanai. Zinātniskie raksti: 2015. gada medicīnas nozares pētnieciskā darba publikācijas: Internā medicīna. Ķirurģija. Medicīnas bāzes zinātnes. Stomatoloģija. Farmācija / Rīgas Stradiņa universitāte. – Rīga: Rīgas Stradiņa universitāte, 2016. –Lpp.310-317
• Rots D, Grasmane A, Kreile M, Zariņa A, Keišs J, Sondore V, Piekuse L. MDR1 gēna polimorfismu rs2032582 un rs1045642 saistība ar hroniska C vīrushepatīta attīstības risku un gaitu. Zinātniskie raksti: 2015. gada medicīnas nozares pētnieciskā darba publikācijas: Internā medicīna. Ķirurģija. Medicīnas bāzes zinātnes. Stomatoloģija. Farmācija / Rīgas Stradiņa universitāte. – Rīga: Rīgas Stradiņa universitāte, 2016. lpp 318-326.

2015

• Zole, E., Elferts, D., Kimsis, J., Krumina, A., Narels, K., Pole, I., Ranka, R., Pliss, L. (2015). Comparison of telomere length between population-specific mitochondrial haplogroups among different age groups in a Latvian population. Mech Ageing Dev, 145, 13-17. doi:10.1016/j.mad.2015.01.002
• Pliss L, Timša L, Rootsi S, Tambets K, Pelnena I, Zole E, Puzuka A, Sabule A, Rozane S, Lace B, Kucinskas V, Krumina A, Ranka R, Baumanis V. Y-Chromosomal Lineages of Latvians in the Context of the Genetic Variation of the Eastern-Baltic Region. Ann Hum Genet. 2015 Nov;79(6):418-30. doi: 10.1111/ahg.12130. Epub 2015 Sep 28.
• Daugule, I., Ruskule, A., Moisejevs, G., Rudzite, D., Jonaitis, L., Janciauskas, D., Kupcinskas, L., Leja, M. (2015). Long-term dynamics of gastric biomarkers after eradication of Helicobacter pylori infection. Eur J Gastroenterol Hepatol, 27(5), 501-505. doi:10.1097/meg.0000000000000308

2014

• Kreile M, Rots D, Piekuse L, Cebura E, Grutupa M, Kovalova Z, Lace B. Lack of association between polymorphisms in genes MTHFR and MDR1 with risk of childhood acute lymphoblastic leukemia. Asian Pac J Cancer Prev. 2014;15(22):9707-11.
• Piekuse L, Kreile M, Zarina A, Steinberga Z, Sondore V, Keiss J, Lace B, Krumina A. Association between inherited monogenic liver disorders and chronic hepatitis C. World J Hepatol. 2014 Feb 27;6(2):92-7. doi: 10.4254/wjh.v6.i2.92.
• Piekuse L., Lace B, Kreile M, Sadovska L, Kempa I, Daneberga Z, Micule I, Sondore V, Keiss J, Krumina A. Impact of the genes UGT1A1, GSTT1, GSTM1, GSTA1, GSTP1 and NAT2 on acute alcohol-toxic hepatitis. Central European Journal of biology, Vol 9 (2), February 2014, pp 125-130. doi: 10.2478/s11535-013-0249-y
• Kevere L, Purvina S, Bauze D, Zeibarts M, Andrezina R, Piekuse L, Brekis E, Purvins I. Homocysteine and MTHFR C677T polymorphism in children and adolescents with psychotic and mood disorders. Nord J Psychiatry. 2014 Feb; 68(2):129-36. doi: 10.3109/08039488.2013.782066. Epub 2013 Apr 16. PMID: 23586533
• Kempa I, Ambrozaitytė L, Stavusis J, Akota I, Barkane B, Krumina A, Matulevičienė A, Utkus A, Kučinskas V, Lace B. Association of BMP4 polymorphisms with non-syndromic cleft lip with or without cleft palate and isolated cleft palate in Latvian and Lithuanian populations. Stomatologija. 2014;16(3):94-101.

2013

• Piekuse, L., Kreile, M., Zarina, A., Shteinberga, Z., Sondore, V., Keiss, J., Lace, B., Krumina, A. Association between inherited monogenic liver disorders and chronic hepatitis C. World Journal of Hepatology (In press).
• Piekuse, L., Lace, B., Kreile, M., Sadovska, L., Kempa, I., Daneberga, Z., Micule, I., Sondore, V., Keiss, J., Krumina, A. Impact of the genes UGT1A1, GSTT1, GSTM1, GSTA1, GSTP1 and NAT2 on acute alcohol-toxic hepatitis. Central European Journal of biology, Vol 9 (2), February 2014, pp 125-130. doi: 10.2478/s11535-013-0249-y
• Kevere, L., Purvina, S., Bauze, D., Zeibarts, M., Andrezina, R., Piekuse, L., Brekis, E., Purvins, I. Homocysteine and MTHFR C677T polymorphism in children and adolescents with psychotic and mood disorders. Nord J Psychiatry. 2014 Feb;68(2):129-36. doi: 10.3109/08039488.2013.782066. Epub 2013 Apr 16. PMID: 23586533\
• Bauze, D., Piekuse, L., Kevere, L., Kronberga, Z., Rizevs, A., Vaivade, I., Viksne, K., Andrezina, R., Lace, B. Association a Single Nucleotide Polymorphism in Chromosome 11 with Autism Spectrum Disorder in a North-Eastern European Population. Proceedings of the Latvian Academy of Science, 2013 (In press).
• Berza, N., Zodzika, J., Kroica, J., Reinis, A., Skadins, I., Piekuse, L., Melngaile, O., Pundure,R., Lukojanova, I., Vasina, O. Association between Lactobacillus species and bacterial vaginosis-related bacteria, and bacterial vaginosis scores in small population of pregnant Latvian women. International Journal of Collaborative Research on Internal Medicine & Public Health, 2013, Vol. 5 No. 5, pp255-264.
• Skesters, A. P., Silova, A. A., Spadiene, A. G., Savickiene, N. Z., Rusakova, N. J., Larmane, L. T., Moisejevs, G. O. Redox status blood of patients with new-found type-2 diabetes mellitus before and after duration of administration of natural antioxidants. Prenosology and Healthy Lifestyle, 1(12), 2013, 76-79 pp.; (in Russian)

2012

• Kevere L., Purvina S., Bauze D., Zeibarts M., Andrezina R., Rizevs A., Jelisejevs S., Piekuse L., Kreile M., Purvins I.: Elevated serum levels of homocysteinedisord as an early prognostic factor of psychiatric disorders in children and adolescents, Schizophr Res Treatment. 2012(2012:373261), pp., 2012, doi:10.1155/2012/373261. Epub, 2012, Oct 2.
• Sadovska L., Piekuse L., Kreile M., Keiss J., Krumina A.: CCR5-32 and UGT1A1 28-mutations in HCV patients, Immunology. Vol. 137 Suppl. 1, pp. 497-497, 2012
• Letra A., Fakhouri W., Fonseca R., Menezes R., Kempa I., Prasad J., McHenry T., Lidral A., Moreno L., Murray J., Daack-Hirsch S., Marazita M., Castilla E., Lace B., Orioli I., Granjeiro J., Schutte B., Vieira A.: Interaction between IRF6 and TGFA Genes Contribute to the Risk of Non syndromic Cleft Lip/Palate, PLoS One. 7(9), 2012, doi:10.1371/journal.pone.0045441
• Lace B., Kempa I., Klovins J., Stavusis J., Krumina A., Akota I., Barkane B., Vieira A., Nagle E., Grinfelde I., Maulina I.: BCL3 gene role in facial morphology, Birth Defects Res. Part A-Clin. Mol. Teratol. Vol. 94(11), pp. 918-924, 2012
• Kreile M., Daneberga Z., Ziemele I., Eglite I., Grinfelde I., Sviridova D., Krumina Z.: Shwachman diamond syndrome mimics disorder of galactose metabolisms, J. Inherit. Metab. Dis.. Vol. 35 Suppl. 1, pp. S166-S166, 2012

2011

• Makrecka, M., Kuka, J., Liepinsh, E., Dambrova, M.: T Nikopensius, T., Kempa, I., Ambrozaityte, L., Jagomagi, T., Saag, M., Matuleviciene, A., Utkus, A., Krjutskov, K., Tammekivi, V., Piekuse, L., Akota, I., Barkane, B., Krumina, A., Klovins, J., Lace, B., Kucinskas, V., Metspalu, A.: Variation in FGF1, FOXE1, and TIMP2 genes is associated with nonsyndromic cleft lip with or without cleft palate. Birth Defects Res. Part A-Clin. Mol. Teratol. Vol. 91, pp. 218–225, 2011
• Lace, B., Kempa, I., Piekuse, L., Grinfelde, I., Klovins, J., Pliss, L., Krumina, A., Vieira, A.R.: Association studies of candidate genes and cleft lip and palate taking into consideration geographical origin. European Journal of Oral Sciences, Vol. 119, pp. 413–417, 2011
• Puzuka, A., Pronina, N, Grinfelde, I, Erenpreiss, J, Lejing, V, Bars, J, Pliss, L, Pelnena, I, Baumanis, V, Krumina, A.: Y chromosome-a tool in infertility studies of Latvian population. Russ. J. Genet. Vol. 47, pp. 347–353, 2011

Equipment

Diagnostic Services

Analyses

Name	Time (working days)*
DNA isolation from peripheral blood with EDTA anticoagulant	10
DNA analyses
Cystic fibrosis – Detection of dF508 and dele 2,3 in CFTR gene	10
Cystic fibrosis – Sequencing of the coding part of the CFTR gene	60
Male infertility (azoospermia) – Analysis of AZFa, AZFb and AZFc regions	20
Inherited thrombophilia – Detection of (FV) Leiden G1691A and (FII) Prothrombin G20210A	20
Risk factors for thrombophilia – Detection of C677T and A1298C in MTHFR gene	20
Myeloproliferative neoplasia – Detection of p.V617F variant in JAK2 gene	60

For other genetic testing options, contact mgzlrsu[pnkts]lv

* Times apply only to paid analyses / times for analyses performed in the framework of a scientific cooperation are longer. All deadlines can be extended due to the current state of emergency.