Kalvis Brangulis

Work experience

Education and training

Achievements

Continuing education a.o. qualification upgrade

Practical courses. FEBS practical and lecture course on modern methods of protein crystallography "BioCrys 2012", Portugal, Oeiras, 2012.

Practical courses​. Oulu university practical course on protein crystallography "From data to structures", Finland, Oulu, 2012.

Practical courses​. EMBO practical course "Computational structural biology – from data to structure to function", UK, Hinxton, 2011.

Practical courses. ​EMBO practical course "High-throughput methods for Protein Production and Crystallization", France, Marseille, 2011.

Practical courses. ​EMBO/EMBL practical course Protein expression, purification and crystallisation, Germany, Hamburg, 2010.

Field of research

• Molecular biology
• Structural biology

Head of study course

Medical biochemistry

Lectured study course

Biochemistry

Biochemistry of Different Pathologies

Cell Metabolism

Medical Biochemistry

Preclinical Dismetabolism

Supervised and reviewed student research papers

Supervised:

Master's theses: 3

qualification papers: 7

Reviewed:

PhD theses: 2

Master's theses: 2

qualification papers: 3

Projects

Researcher, ERDF grant Nr. 1.1.1.2/VIAA/1/16/144 "Structural and functional studies of Lyme disease agent Borrelia burgdorferi outer surface proteins to reveal the mechanisms of pathogenesis with the intention to create a new vaccine".

Researcher, EC 7th Framework Programme project REGPOT-CT-2013-316149-InnovaBalt, „Strengthening the research and innovative capacities of the Latvian Institute of Organic Synthesis, the leading Baltic regional centre for drug discovery".

Research assistant, ERDF grant 2DP/2.1.1.1.0/10/APIA/VIAA/022, „Development and validation of novel DNA tests for diagnosis applicable in clinical practice".

Research assistant, ​ESF grant 1DP/1.1.1.2.0/09/APIA/VIAA/150, „The investigation of molecular and genetic mechanisms of pathogenesis and the development of novel means for diagnosis and therapy".

Supervised study course

Medical Biochemistry

Publications

Research articles​​

Crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent Borrelia burgdorferi / K.Brangulis, I.Akopjana, A.Kazaks, K.Tars // Acta Crystallographica. Sect.D. Structural Biology. - Vol.75, Part 9 (2019, Sept.), p.825-830. - Web of Science un/vai Scopus. - DOI: 10.1107/S2059798319010751

Crystal structure of Borrelia burgdorferi outer surface protein BBA69 in comparison to the paralogous protein CspA / K.Brangulis, I.Akopjana, I.Petrovskis ...[u.c.] // Ticks and Tick-borne Diseases. - Vol.10, No.5 (2019, Aug.), p.1135-1141. - Web of Science un/vai Scopus. - 10.1016/j.ttbdis.2019.06.009

Crystal structure of the membrane attack complex assembly inhibitor BGA71 from Lyme disease agent Borrelia bavariensis [Elektroniskais resurss] / K.Brangulis, I.Akopjana, I.Petrovskis ...[et al.] // Scientific Reports [Elektroniskais resurss]. - Vol.8 (2018, July), article No.11286, [11 p.]. - Pieejas veids: Tīmeklis WWW.URL: https://www.nature.com/articles/s41598-018-29651-9.pdf

Crystal structures of the Erp protein family members ErpP and ErpC from Borrelia burgdorferi reveal the reason for different affinities for complement regulator factor H. / K.Brangulis, I.Petrovskis, A.Kazaks ...[et al.] // Biochimica et Biophysica Acta. - Vol.1854, No.4 (2015, May), p.349-355. - Starptautiski citējamā izdevumā.

Structural and functional analysis of BB0689 from Borrelia burgdorferi, a member of the bacterial CAP superfamily / K.Brangulis, K.Jaudzems, I.Petrovskis ...[et al.] // Journal of Structural Biology. - Vol.192, No.3 (2015, Dec.), p.320-330. - Starptautiski citējamā izdevumā.

Crystal structure of the infectious phenotype-associated outer surface protein BBA66 from the Lyme disease agent Borrelia burgdorferi / K.Brangulis, I.Petrovskis, R.Ranka...[et al.] // Ticks and Tick-borne Diseases. - Vol.5, No.1 (2014, Febr.), p.63-68. - Starptautiski citējamā izdevumā.

Structural characterization of CspZ, a complement regulator factor H and FHL-1 binding protein from Borrelia burgdorferi / K.Brangulis, I.Petrovskis, R.Ranka ...[et al.] // The FEBS Journal. - Vol.281, No.11 (2014, June), p.2613-2622. - Starptautiski citējamā izdevumā.

Analysis of seroreactivity to recombinant B. burgdorferi antigens BBA65 and BBA73 / R.Ranka, K.Brangulis, D.Bandere ...[et al.] // Collection of Scientific Papers 2014 : Research articles in medicine & pharmacy / Rīga Stradiņš University. - Riga, 2014. - P.40-46.

Fibronectin-binding nanoparticles for intracellular targeting addressed by B. burgdorferi BBK32 protein fragments / R.Ranka, K.Brangulis, I.Petrovskis ...[et al.] // Nanomedicine : Nanotechnology, Biology, and Medicine. - Vol.9, No.1 (2013, Jan.), p.65-73. - Starptautiski citējamā izdevumā.

Structural characterization of the Borrelia burgdorferi outer surface protein BBA73 implicates dimerization as a functional mechanism / K.Brangulis, I.Petrovskis, A.Kazaks ...[et al.] // Biochemical and Biophysical Research Communications. - Vol.434, No.4 (2013, May), p.848-853. - Starptautiski citējamā izdevumā.

Structure of an outer surface lipoprotein BBA64 from the Lyme disease agent Borrelia burgdorferi which is critical to ensure infection after a tick bite / K.Brangulis, K.Tars, R.Ranka ...[et al.] // Acta Crystallographica. Sect.D. Biological Crystallography. - Vol.69, No.6 (2013, June), p.1099-1107. - Starptautiski citējamā izdevumā.

Abstracts​​

Brangulis, Kalvis. Crystal structure of Borrelia burgdorferi outer surface protein BBA69 / K.Brangulis // 4th Instruct Biennial Structural Biology Conference "Structural biology: deeper into the cell" (Madrid, Spain, May 22-24, 2019) : Abstract Booklet. - Madrid, 2019. - Poster No.60, p.100.

Crystal structure of BB0365 from Lyme agent Borrelia burgdorferi / K.Brangulis, I.Akopjana, I.Petrovskis ...[et al.] // 13th International Symposium on Ticks and Tick-Borne Diseases (Weimar, Germany, March 28-30, 2019) : Programme & Abstract Book. - Weimar, 2019. - P.84.

Crystal structure of Borrelia burgdorferi outer surface protein BBK01 – a member of the paralogous gene family 12 / K.Brangulis, I.Akopjana, A.Kazaks, K.Tars // FEBS Open Bio. - Vol.9, Suppl.1 (2019, July), poster No.P-27-064, p.267. - The 44th FEBS Congress (Krakow, Poland, July 6-11, 2019) : Posters.

Crystal structure of the membrane attack complex assembly inhibitor BGA71 from Lyme disease agent Borrelia bavariensis [Elektroniskais resurss] / K.Brangulis, I.Akopjana, I.Petrovskis ...[et al.] // FEBS Open Bio [Elektroniskais resurss]. - Vol.8, Suppl.1 (2018, July), p.441. - 43rd FEBS Congress "Biochemistry forever" (Prague, Czech Republik, July 7-12, 2018) : Abstracts. - Pieejas veids: Tīmeklis WWW.URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031936/pdf/FE…- Starptautiski citējamā izdevumā.

The crystal structure of Lyme disease agent Borrelia burgdorferi surface protein BBE31 that is vital for successful colonization of a mammalian host [Elektroniskais resurss] / K.Brangulis, I.Akopjana, I.Petrovskis ...[et al.] // The FEBS Journal [Elektroniskais resurss]. - Vol.284, Issue Suppl.S1 (2017, Sept.), p.132-133. - Starptautiski citējamā izdevumā. - Pieejas veids: Tīmeklis WWW.URL: http://onlinelibrary.wiley.com/doi/10.1111/febs.14174/epdf

Study of B. burgdorferi outer membrane proteins by protein-protein interaction approach / R.Ranka, K.Brangulis, V.Baumanis ...[et al.] // The FEBS Journal. - Vol.280, Suppl.1 (2013, July.), p.490. - Starptautiski citējamā izdevumā.

Successful creation of chimeric virus-like particles maintaining fibronectin-binding property from B. burgdorferi BBK32 protein [Elektroniskais resurss] : [abstract] / R.Ranka, K.Brangulis, V.Baumanis ...[et al.] // XII International Jena Symposium on Tick-borne Diseases (Weimar, Germany, March 21-23, 2013) [Elektroniskais resurss]. - Weimar, 2013. - Poster No.59. - Resurss aprakstīts 2014.g. 27.febr. - Pieejas veids: Tīmeklis WWW.URL: http://www.tbd-symposium.com/media/public/abs_ijstd_xii/P59…

Objects of intellectual property rights

Coupling of Borrelial protein BB0689 to virus-like particles for generation of Lyme disease vaccine : EP3135295 (A1) : international patent / inventors: K.Tars, I.Petrovskis, K.Brangulis ...[et al.]. - Filing date: 28.08.2015 ; publication date: 01.03.2017.